Pyrophosphate: fructose 6-phosphate phosphotransferase in germinating castor bean seedlings

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Kinetic properties of pyrophosphate:fructose-6-phosphate phosphotransferase from germinating castor bean endosperm.

Pyrophosphate:fructose-6-phosphate phosphotransferase (PFP) was purified over 500-cold from endosperm of germinating castor bean (Ricinus commiunis L. var. Hale). The kinetic properties of the purified enzyme were studied. PFP was specific for pyrophosphate and had a requirement for a divalent metal ion. The pH optimum for activity was 7.3 to 7.7. The enzyme had similar activities in the forwar...

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During germination and early growth of the castor bean (Ricinus communis) nitrogenous constituents from the endosperm are transferred via the cotyledons to the growing embryo. Exudate collected from the cut hypocotyl of 4-day seedlings contained 120 millimolar soluble amino nitrogen and glutamine was the predominant amino acid present, comprising 35 to 40% of the total amino nitrogen. To determ...

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Lectins in castor bean seedlings.

The amounts of the two lectins (ricin and Ricinus communis agglutinin) in tissues of castor bean seedlings were followed during germination and early growth. For measurement, lectins in extracts were separately eluted from Sepharose columns; an antibody to the agglutinin was also used to detect the lectins by immunodiffusion. The endosperm of the dry seed contains 3.5 mg total lectin (5.6% of t...

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Fructose - l , 6 - Bisphosphate 1 s an Allosteric Activator of Pyrophosphate : Fructose - 6 - Phosphate 1 - Phosphotransferase ' Tom

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ژورنال

عنوان ژورنال: FEBS Letters

سال: 1983

ISSN: 0014-5793

DOI: 10.1016/0014-5793(83)80654-1